Comparison of Segmentation Analysis in Nucleus Detection with GLCM Features using Otsu and Polynomial Methods
Abstract
Pap smear is a digital image generated from the recording of cervical cancer cell preparation. Images generated are susceptible to errors due to the relatively small cell sizes and overlapping cell nuclei. Therefore, accurate Pap smear image analysis is essential to obtain the right information. This research compares nucleus segmentation and detection using Grey Level Co-occurrence Matrix (GLCM) features in two methods: Otsu and Polynomial. The tested data consisted of 400 images sourced from RepoMedUNM, a publicly accessible repository containing 2,346 images. Both methods were compared and evaluated to obtain the most accurate features. The research results showed that the average distance of the Otsu method was 6.6457, which was superior to the Polynomial method with a value of 6.6215. Distance refers to the distance between the nucleus detected by the Otsu and the Polynomial method. Distance is an important measure to assess how closely the detection results align with the actual nucleus positions. It indicates that the Polynomial method produces nucleus detections that are on average closer to the actual nucleus positions compared to the Otsu method. Consequently, this research can serve as a reference for further studies in developing new methods to enhance the accuracy of identification.
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References
D. Riana, M. Jamil, S. Hadianti, J. Na’am, H. Sutanto, and R. Sukwadi, “Model of Watershed Segmentation in Deep Learning Method to Improve Identification of Cervical Cancer at Overlay Cells,” TEM Journal, pp. 813–819, May 2023, doi: 10.18421/tem122-26
[2] R. Kavitha et al., “Ant Colony Optimization-Enabled CNN Deep Learning Technique for Accurate Detection of Cervical Cancer,” BioMed Research International, vol. 2023, pp. 1–9, Feb. 2023, doi: 10.1155/2023/1742891
G. Duru and S. Topatan, “A barrier to participation in cervical cancer screenings: fatalism,” Women & Health, vol. 63, no. 6, pp. 436–444, Jun. 2023, doi: 10.1080/03630242.2023.2223698.
G. Song, J. Han, Y. Zhao, Z. Wang, and H. Du, “A Review on Medical Image Registration as an Optimization Problem,” Current Medical Imaging Reviews, vol. 13, no. 3, Jul. 2017, doi: 10.2174/1573405612666160920123955.
J. Bierbrier, H.-E. Gueziri, and D. L. Collins, “Estimating medical image registration error and confidence: A taxonomy and scoping review,” Medical Image Analysis, vol. 81, p. 102531, Oct. 2022, doi: 10.1016/j.media.2022.102531.
R. Di Fiore et al., “Cancer Stem Cells and Their Possible Implications in Cervical Cancer: A Short Review,” International Journal of Molecular Sciences, vol. 23, no. 9, p. 5167, May 2022, doi: 10.3390/ijms23095167.
D. Riana, A. N. Hidayanto, and Fitriyani, “Integration of Bagging and greedy forward selection on image pap smear classification using Naïve Bayes,” 2017 5th International Conference on Cyber and IT Service Management (CITSM), Aug. 2017, doi: 10.1109/citsm.2017.8089320.
W. Liu et al., “CVM-Cervix: A hybrid cervical Pap-smear image classification framework using CNN, visual transformer and multilayer perceptron,” Pattern Recognition, vol. 130, p. 108829, Oct. 2022, doi: 10.1016/j.patcog.2022.108829.
H. L. Glasgow et al., “Laminin targeting of a peripheral nerve-highlighting peptide enables degenerated nerve visualization,” Proceedings of the National Academy of Sciences, vol. 113, no. 45, pp. 12774–12779, Oct. 2016, doi: 10.1073/pnas.1611642113.
J. Pulkkinen, H. Huhtala, and I. Kholová, “False-positive atypical endocervical cells in conventional Pap smears: Cyto-histological correlation and analysis,” Acta Cytologica, Aug. 2023, doi: 10.1159/000533256.
N. Nahrawi et al., “A review of detection and classification cervical cell images,” AIP Conference Proceedings, 2023, doi: 10.1063/5.0127798.
Nur Ain Alias, Wan Azani Mustafa, Mohd Aminudin Jamlos, Shahrina Ismail, Hiam Alquran, and Mohamad Nur Khairul Hafizi Rohani, “Pap Smear Image Analysis Based on Nucleus Segmentation and Deep Learning – A Recent Review,” Journal of Advanced Research in Applied Sciences and Engineering Technology, vol. 29, no. 3, pp. 37–47, Feb. 2023, doi: 10.37934/araset.29.3.3747.
I. Pacal and S. Kılıcarslan, “Deep learning-based approaches for robust classification of cervical cancer,” Neural Computing and Applications, vol. 35, no. 25, pp. 18813–18828, Jul. 2023, doi: 10.1007/s00521-023-08757-w.
W. William, A. Ware, A. H. Basaza-Ejiri, and J. Obungoloch, “A review of image analysis and machine learning techniques for automated cervical cancer screening from pap-smear images,” Computer Methods and Programs in Biomedicine, vol. 164, pp. 15–22, Oct. 2018, doi: 10.1016/j.cmpb.2018.05.034.
Y. Hamama-Raz, S. Shinan-Altman, and I. Levkovich, “The intrapersonal and interpersonal processes of fear of recurrence among cervical cancer survivors: a qualitative study,” Supportive Care in Cancer, vol. 30, no. 3, pp. 2671–2678, Nov. 2021, doi: 10.1007/s00520-021-06695-8.
M. E. Plissiti, P. Dimitrakopoulos, G. Sfikas, C. Nikou, O. Krikoni, and A. Charchanti, “Sipakmed: A New Dataset for Feature and Image Based Classification of Normal and Pathological Cervical Cells in Pap Smear Images,” 2018 25th IEEE International Conference on Image Processing (ICIP), Oct. 2018, doi: 10.1109/icip.2018.8451588.
R. Guido, “Secondary Prevention of Cervical Cancer Part 2,” Clinical Obstetrics & Gynecology, vol. 57, no. 2, pp. 292–301, Jun. 2014, doi: 10.1097/grf.0000000000000033.
I. Rodriguez et al., “Insights into the Mechanisms and Structure of Breakage-Fusion-Bridge Cycles in Cervical Cancer using Long-Read Sequencing,” Aug. 2023, doi: 10.1101/2023.08.21.23294276.
Y. Li, F. Chen, J. Shi, Y. Huang, and M. Wang, “Convolutional Neural Networks for Classifying Cervical Cancer Types Using Histological Images,” Journal of Digital Imaging, vol. 36, no. 2, pp. 441–449, Dec. 2022, doi: 10.1007/s10278-022-00722-8.
F. Kuang, J. Ren, Q. Zhong, F. Liyuan, Y. Huan, and Z. Chen, “The value of apparent diffusion coefficient in the assessment of cervical cancer,” European Radiology, vol. 23, no. 4, pp. 1050–1058, Nov. 2012, doi: 10.1007/s00330-012-2681-1.
J. Jordan et al., “European guidelines for quality assurance in cervical cancer screening: recommendations for clinical management of abnormal cervical cytology, part 1,” Cytopathology, vol. 19, no. 6, pp. 342–354, Dec. 2008, doi: 10.1111/j.1365-2303.2008.00623.x.
H. L. Glasgow et al., “Laminin targeting of a peripheral nerve-highlighting peptide enables degenerated nerve visualization,” Proceedings of the National Academy of Sciences, vol. 113, no. 45, pp. 12774–12779, Oct. 2016, doi: 10.1073/pnas.1611642113.
S. Zhang, Z. Ma, G. Zhang, T. Lei, R. Zhang, and Y. Cui, “Semantic image segmentation with deep convolutional neural networks and quick shift,” Symmetry (Basel), vol. 12, no. 3, pp. 1–11, 2020. doi: 10.3390/sym12030427.
Y. H. Tao Wang, Jinjie Huang , Dequan Zheng, “Nucleus segmentation of cervical cytology images based on depth information,” IEE Access, vol. 8, pp. 75846–75859, May 2020, doi: 10.1109/ACCESS.2020.2989369.
R. Zou et al., “Effects of metalloprotease ADAMTS12 on cervical cancer cell phenotype and its potential mechanism,” Discover Oncology, vol. 14, no. 1, Aug. 2023, doi: 10.1007/s12672-023-00776-2.
J. C. LeCher et al., “Utilization of a cell‐penetrating peptide‐adaptor for delivery of human papillomavirus protein E2 into cervical cancer cells to arrest cell growth and promote cell death,” Cancer Reports, vol. 6, no. 5, Mar. 2023, doi: 10.1002/cnr2.1810.
A. Saihood, H. Karshenas, and A. R. Naghsh-Nilchi, “Multi-Orientation Local Texture Features for Guided Attention-Based Fusion in Lung Nodule Classification,” IEEE Access, vol. 11, pp. 17555–17568, 2023, doi: 10.1109/access.2023.3243104.
D. Riana et al., “RepoMedUNM: A New Dataset for Feature Extraction and Training of Deep Learning Network for Classification of Pap Smear Images,” in International Conference on Neural Information Processing, 2021, pp. 317–325, doi: 10.1007/978-3-030-92307-5_37.
M. Wati, D. Dwiani Samjar, H. Haviluddin, and F. Alameka, “Identifikasi Senjata Tradisional Mandau Suku Dayak Menggunakan Metode Support Vector Machine,” Metik J., vol. 6, no. 1, pp. 70–78, 2022, doi: 10.47002/metik.v6i1.341.
W. Thalib, A. Luhur, and M. W. Paryasto, “Analisis Berat Dan Ukuran Telur Ayam Menggunakan Metode Otsu Berbasis Citra Digital,” e-Proceeding Eng., vol. 10, no. 1, pp. 189–195, 2023.
Hong, G., Luo, M. R., & Rhodes, P. A. “Colorimetric Characterization Based on Polynomial Modeling”. Time, 2(Iso 17321), 2000
N. Merlina, E. Noersasongko, P. N. Andono, M. A. Soeleman, D. Riana, and J. Na, “Medical Image Registration at Pap Smear for Early Identification of Cervical Cancer,” TEM J., vol. 12, no. 2, pp. 726–731, 2023, doi: 10.18421/TEM122.
N. Merlina, E. Noersasongko, P. Nurtantio, M. A. Soeleman, D. Riana, and S. Hadianti, “Detecting the Width of P p S mear Cytoplasm Image Based on GLCM Feature,” Y.-D. Zhang et al. (eds.), Smart Trends in Computing and Communications: Proceedingsof SmartCom 2020, Smart Innovation, Systems and Technologies 182,https://doi.org/10.1007/978-981-15-5224-3_22.
F. Aziz, F. Ernawan, M. Fakhreldin, and P. W. Adi, “YOLO Network-Based for Detection of Rice Leaf Disease,” 2023 International Conference on Information Technology Research and Innovation (ICITRI), Aug. 2023, doi: 10.1109/icitri59340.2023.10249843.
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